Identify the proteins that form complexes with PMCA and CaM in the raft vs non-raft domains from F344/BNF1 rat brain at 5-mos of age.
Determine if the proteins that form complexes with PMCA and CaM in raft vs non-raft domains differ in brain preparations from 5 versus 34 mos old rats.
Analyze raft preparations from 5 and 34 mos old rats for post-translational markers of exposure to reactive nitrogen species (RNS).
In collaboration with Core B, create expansive, well-annotated database of the rat synaptic membrane and neuronal raft proteome to be used in highly quantitative assessments of protein differences in preparations from 5 versus 34 mos old rats.
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